Non-Human DHPS Models

Non-human DHPS models include Neurospora crassa, Leishmania donovani, T. brucei, and bovine DHPS.

• Allosteric activation of trypanosomatid deoxyhypusine synthase by a catalytically dead paralog. (PMID: 23525104,(ncbi.nlm.nih.gov)) Nguyen S. … Phillips M.A. J. Biol. Chem. 2013 3 64
  • Mammalian blood form  brucei DHPS is activated by a prozyme (catalytically dead paralog) mechanism.
• Identification and characterization of a novel deoxyhypusine synthase in Leishmania donovani. (PMID: 19880510,(ncbi.nlm.nih.gov)) Chawla B. … Madhubala R. J. Biol. Chem. 2010 22 64
  • A protozoan parasite has a different version of the DHPS than humans, and two putative DHPS sequences have been identified in its genome. The differences between the two enzymes could be exploited for drug development for diseases caused by protozoan parasites.
• Molecular cloning of bovine eIF5A and deoxyhypusine synthase cDNA. (PMID: 15354351,(ncbi.nlm.nih.gov)) Huang J.K. … Wen L. DNA Seq. 2004 22 64
  • Here we describe the cloning and characterization of bovine eIF5A and bovine DHPS, which showed a 93% identity to the human protein.
• Higher activity of recombinant bovine deoxyhypusine synthase vs. human deoxyhypusine synthase. (PMID: 15039063,(ncbi.nlm.nih.gov)) Huang J.K. … Wen L. Protein Expr. Purif. 2004 22 64
  • Bovine DHPS has considerably higher activity than human DHPS in catalyzing the synthesis of deoxyhypusine.
• Molecular cloning and functional expression of human deoxyhypusine synthase cDNA based on expressed sequence tag information. (PMID: 8615810,(ncbi.nlm.nih.gov)) Yan Y.P. … Chen K.Y. Biochem. J. 1996 3 4 22 64
  • Researchers cloned and characterized the Neurospora crassa DHPS cDNA using a reverse genetics approach.